The Infona portal uses cookies, i.e. strings of text saved by a browser on the user's device. The portal can access those files and use them to remember the user's data, such as their chosen settings (screen view, interface language, etc.), or their login data. By using the Infona portal the user accepts automatic saving and using this information for portal operation purposes. More information on the subject can be found in the Privacy Policy and Terms of Service. By closing this window the user confirms that they have read the information on cookie usage, and they accept the privacy policy and the way cookies are used by the portal. You can change the cookie settings in your browser.
Rotation manipulation of Caenorhabditis elegans (C. elegans) is presented for higher precision by a microchannel. The C. elegans was trapped within a biocompatible gel bead to rotate by a rotational motor. An alginate was used to form the gel bead. Without immobilization treatment by a sodium azide, the C. elegans moves during solidification of alginate. Then, a cavity was happened in the alginate...
This study presents the high-precision microinjection of fluorescent micro-gel beads into Caenorhabditis elegans trapped in a suction microchannel. In our previous works, we demonstrated survival microinjection by a conventional micromanipulation technique. However, the focal planes differed between the tip of the microinjection tool and the target axon inside the C. elegans body. To resolve this...
In this paper, we propose and demonstrate a new method for local cell stiffness measurement using multiple probes system. Thin glass pipettes were employed to measure the stiffness of cells on a general culture dish. The thin micropipettes were prepared and their spring constant was measured using the Atomic Force Microscopy (AFM) cantilever before stiffness measurement. The deformation of the pipette...
Plasticizer effect plays an important role in biological field, such as in the fruit ripe process. Humidity is a key factor to affect the plasticize. Previously, the stiffness of plant cells have been measured to study the plasticizer of the plant. However, these studies are based on group cell analysis and is not in vivo. In this paper, A nanoneedle was fabricated from AFM (atomic force microscopy)...
Single cell's mechanical property characterization is important for the understanding of the cell's condition and activity. In this paper, an automatic nanomanipulation system was proposed for single cell mechanical property characterization inside an environmental scanning electron microscopy (ESEM). A nanoneedle was fabricated from AFM (atomic force microscopy) cantilever by FIB (focused ion beam)...
In this paper, we try to establish new technique that the components of the biological clock are reconstituted into the liposome. In other words, we try to produce a nano size clock, capsulated into the liposome, made by protein molecules. The circadian clock is a basic cellular system found in almost all organisms. This clock generates self-sustained oscillations under constant conditions with a...
Toward to realizing establishment of new micro robot for micro-nano surgery, drag delivery etc., many people proposed microbe as actuator. So, evaluation of microbe property for these application arias is important. From this viewpoint, we try to fabricate evaluation tool for bacterial behavior in micro channel like capillary vessel. This evaluation will be helpful to our recent study “bacteria driven...
In this paper, we established the driving systems for micro structures by using surface swarming of Vibrio alginolyticus. First, we succeeded in causing rotational movement using ratchet shape micro channel. Next, To rectify rotational movement, we fabricated center prior in the micro channel. We succeeded in smooth rotational movement using this new design micro channel. Furthermore, we succeeded...
This paper presents a novel selective nano-injection method by fluorescent nanoprobe insertion based on Nanomanipulation under hybrid microscope. The hybrid microscope is designed to combine the optical microscope (OM) and environmental-scanning electron microscope (E-SEM) to realize biological specimen analysis by optical imaging including fluorescent imaging, and nano-scale manipulation by E-SEM...
In this paper, we try to establish newly technique that the components of the biological clock are reconstituted into the liposome. In other words, we try to produce a nano size clock, capsulated into the liposome, made by protein molecules. The circadian clock is a basic cellular system found in almost all organisms. This clock generates self-sustained oscillations under constant conditions with...
Toward to realizing establishment of new micro robot for micro-nano surgery, drag delivery etc., many people proposed microbe as actuator. So, evaluation of microbe property for these application arias is important. In this paper, we try to fabricate evaluation tool for bacterial behavior in micro channel like capillary vessel. For this purpose, we fabricated line patterned PDMS chip and use these...
In this paper, we established the transport systems for micro object by using surface swarming of Vibrio alginolyticus. First, we confirmed that V. alginolyticus were able to transport the micro beads. In this experiment, we used YM19 and YM19ΔcheY that were a kind of the variant strain of V. alginolyticus appropriate for surface swarming. Next, we examined relationship between the transport speed...
Set the date range to filter the displayed results. You can set a starting date, ending date or both. You can enter the dates manually or choose them from the calendar.