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Purpose: Ile or Val is sometimes seen on the [alpha ]-helices in natural proteins, although [beta ]-branched amino acids destabilize [alpha ]-helices. To clarify the role of these [beta ]-branched amino acids, we examined the effect of replacing Ile by Leu in a natural [alpha ]-helical protein.Methods: The third repeat of the DNA-binding domain of mouse c-Myb composed of three helices, each of which...
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