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Purpose: Determination of the effect of 4, 4[prime ]-diisothiocyano-2, 2[prime ]-stilbene disulfonate (DIDS) on the mitochondrial ADP/ATP carrier (AAC).Methods: Bovine heart mitochondria and their inverted vesicles were treated with various concentrations of DIDS for 10 min at 25[deg ]C.Results: DIDS inhibited ADP uptake via AAC of inverted vesicles, but not mitochondria. Of the mitochondrial membrane...
Purpose: Determination of the functional properties of mammalian hexokinase. For this, we examined its reactivities with ATP molecules by measuring its ATPase activity.Methods: The catalytic domain (C-terminal half) of rat type II hexokinase (50kDa) was expressed in a pET system. The expressed enzyme was purified by ion-exchange (HiTrapQ, Pharmacia) and gel-filtration chromatographies.Results and...
The authors used a biosensor based on surface plasmon resonance (SPR) to perform kinetic measurements of the interaction between an oligosaccharide and various lectins. A glycopeptide, prepared from asialofetuin and having a nearly homogeneous N-linked sugar chain, was immobilized on the surface of a sensor chip via the amino groups of its peptide moiety. The interactions of this bound glycopeptide...
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