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A method allowing simultaneous genotyping of two loci: pre-implantation protein 3 (prei3) and estrogen receptor (ESR) was presented. In multiplex PCR amplification, two amplicons were simultaneously produced a 294 bp fragment of prei3 gene and a 121 bp fragment of ESR gene and were then subjected to “one-tube” restriction enzyme digestion with MspI and PvuII. A total of 92 sows from the 4th dam line...
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