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A DNA fragment (4262bp) of sheep GPR54 gene was obtained through PCR and DNA sequencing methods. One nucleotide transition mutation (A125G) and one 5bp deletion/insertion (TTCTT) mutation at the 163–167 locus in 5´-regulatory region of sheep GPR54 gene were identified by sequence alignment between Small Tail Han and Corriedale sheep. The distributions of these two mutations were determined in high...
The kisspeptin/GPR54 pathway is crucial in the process of puberty onset. Six pairs of primers were designed to clone goat GPR54 and scan polymorphisms and one pair of primers to detect polymorphisms of GPR54 in sexual precocious and sexual late-maturing goat breeds. A DNA fragment of 4258 bp of goat GPR54 was obtained, which contains an open reading frame (ORF) of 1137 bp and encodes 378 amino acids,...
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