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We have performed clinical translation of epithelial cell sheets fabricated on temperature-responsive culture surfaces to treat cornea and esophagus. In the preclinical study using animal models, we found epithelial cell growth potential varied among species. Canine epithelial cell growth was prominent, while rat one was poor under 3T3 feeder layer-free condition. The aim of the present study was...
Temperature-responsive culture dishes immobilized with insulin have been fabricated and studied to shorten cell culture periods by facilitating more rapid cell proliferation. Cells are recovered as contiguous cell sheets simply by temperature changes. Functionalized culture dishes were prepared by previously reported electron beam grafting copolymerization of N-isopropylacrylamide (IPAAm) with its...
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