Fluorinated nucleotides are invaluable for
19F NMR studies of nucleic acid structure and function. Here, we synthesized 4′‐SCF
3‐thymidine (T
) and incorporated it into DNA by means of solid‐phase DNA synthesis. NMR studies showed that the 4′‐SCF
3 group exhibited a flexible orientation in the minor groove of DNA duplexes and was well accommodated...