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IntroductionDissolved free amino acids (DFAA) in intracellular extracts of marine microalgae can be determined by sequential injection chromatography (SIC). This technique uses portable, low‐cost instrumentation but its applications have been limited to short monolithic columns because of components not resistant to high pressures.
ObjectiveTo develop a SIC method for determination of DFAA by exploring...
The concept of sequential injection chromatography (SIC) was exploited to automate the fluorimetric determination of amino acids after pre-column derivatization with o-phthaldialdehyde (OPA) in presence of 2-mercaptoethanol (2MCE) using a reverse phase monolithic C 18 stationary phase. The method is low-priced and based on five steps of isocratic elutions. The first step employs the mixture...
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