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Abstract. A novel fluorescent cytochemical method for sialidase activity was developed using 5-bromo-4-chloroindol-3-yl--D-N-acetylneuraminic acid (X-Neu5Ac) as the substrate. Intact nuclei were isolated from porcine liver and incubated at 37C for 3h with 1mM X-Neu5Ac at pH4.8. The nuclei were stained with blue color that was derived from the oxidized compound of the reaction productX (5-bromo-4-chloro-3-hydroxyindole)...
. A novel fluorescent cytochemical method for sialidase activity was developed using 5-bromo-4-chloroindol-3-yl-α-D-N-acetylneuraminic acid (X-Neu5Ac) as the substrate. Intact nuclei were isolated from porcine liver and incubated at 37°C for 3 h with 1 mM X-Neu5Ac at pH 4.8. The nuclei were stained with blue color that was derived from the oxidized compound of the reaction product X (5-bromo-4-chloro-3-hydroxyindole)...
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