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We study the phase behavior when a mixture of two semi-flexible polymers, giant DNA and alginate, is highly confined mixture in a cell-sized scale space. The characteristic lengths of giant DNA is different from those of alginate. Through the microscopic observation, we found that the volume/surface area ration and the higher-order structure of giant semi-flexible polymer are the crucial determinants...
In this paper, we try to establish newly technique that the components of the biological clock are reconstituted into the liposome. In other words, we try to produce a nano size clock, capsulated into the liposome, made by protein molecules. The biological clock is a basic cellular system found in almost all organisms. This clock generates self-sustained oscillations under constant conditions with...
In this paper, we propose a thermal gel actuated probe which uses the volume change of the thermal gel as an actuator. The thermal gel actuated probe can be used for single cell analysis, especially single cell manipulation. The phase change from sol to gel causes an increase in volume, while the change from gel to sol causes a decrease in volume. The thermal gel actuated probe can spout and suck...
In this paper, we propose a semi-closed microchip which can realize the probe type manipulation in the microchip with the internal environment sealed off from the external environment. Long-term experiments can be conducted for in-situ culture and analysis of cells by the semi-closed microchip. The semi-closed microchip has a bath in the middle of microchannel to allow the insertion of micropipettes...
We propose a nano size clock, capsulated into micro size liposome, made by protein molecules. The circadian clock is a basic cellular system found in almost all organisms. This clock generates self-sustained oscillations under constant conditions with a ??24-hour (circadian) period. In cyanobacteria, circadian clock was reconstituted by only three proteins. We reconstitute these proteins and adenosine...
In this paper, we propose a semi-closed microchip which can realize probe type manipulation in the microchip with the internal environment sealed off from the external environment. The semi-closed microchip has a bath in the middle of microchannel to allow the insertion of micropipettes into the microchip. The bath is sealed off by thin oil film to prevent the evaporation of the solution in the bath...
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