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Images of bacterial cells stained with KK114 dye and visualized with STED microscopy. On the monitor: large field of view of B. subtilis cells, the KK114 ball‐and‐stick model and the schematics of the STED setup. Different space‐filling representations of the FtsZ protein are also shown.
Further details can be found in the article by Massimiliano Lucidi, Radu Hristu, Lorenzo Nichele, George A. Stanciu,...
Super‐resolution microscopy techniques can provide answers to still pending questions on prokaryotic organisms but are yet to be used at their full potential for this purpose. To address this, we evaluate the ability of the rhodamine‐like KK114 dye to label various types of bacteria, to enable imaging of fine structural details with stimulated emission depletion microscopy (STED). We assessed fluorescent...
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