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The detection of induced proteins after introduction of specific substrates in culture is of high interest for a comparative description of organisms growing under different conditions. In this study, protein‐based stable isotope probing (Protein‐SIP) is used for a fast and reliable detection of newly synthesized proteins in a substrate shift experiment. Therefore, Pseudomonas putida ML2 cells precultured...
The structure and function of a microbial community capable of biodegrading methyl‐tert‐butyl ether (MTBE) was characterized using compound‐specific stable isotope analysis (CSIA), clone libraries and stable isotope probing of proteins (Protein‐SIP). The enrichment culture (US3‐M), which originated from a gasoline‐impacted site in the United States, has been enriched on MTBE as the sole carbon source...
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