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It was investigated whether positive immunomagnetic selection with two novel DC-specific mAb allowed purification of functional myeloid dendritic cells (MDC) and plasmacytoid dendritic cells (PDC) from the human lymph nodes (LN). The results were compared with enrichment of DC by low-density Nycodenz gradient centrifugation followed by immunomagnetic depletion of residual B- and T-cells (Nycodenz...
Introduction: The final maturation of dendritic cells (DC), reflected by the upregulation of costimulatory molecules and the enhanced ability of DC to prime naive Th cells, is initiated by locally released inflammatory mediators, including TNFα.We addressed the question of whether the character of the maturation-inducing inflammatory environment may have an impact on the ability of DC to produce IL-12...
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