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Eomesodermin (Eomes) is a transcription factor essential for trophoblast development. Stress stimuli activate stress‐activated protein kinase (MAPK8/9) and modulate transcription factors in trophoblast stem cells (TSC). In this study, we test the hypothesis that stress‐induced Eomes upregulation and downstream trophoblast development are MAPK8/9‐dependent. Immunocytochemical and immunoblot assays...
Accumulating data suggest that 20% O 2 causes human and mouse placental trophoblast stem cell (TSC) differentiation and suppresses proliferation. We tested the hypotheses that phosphorylated stress-activated protein kinase (pSAPK) levels report the optimal O 2 level for TSC culture, and that pSAPK responds to contradictory signals. We tested the dose range of 0–20% O 2 (0,...
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