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DNA methylation plays a key role in the regulation of gene expression. The most common type of DNA modification consists of the methylation of cytosine in the CpG dinucleotide. The detections of DNA methylation have been determined mostly by experimental methods; however, these methods were time-consuming, expensive, and difficult to meet the requirements of modern large-scale sequencing technology...
A convenient method is proposed for precise investigation of the asynchronous structural transition of the domains in bovine serum albumin (BSA) during unfolding process. The method is based on a site-selective probe, alizarin red S (ARS), which has a high affinity to the subdomain IIA of BSA. BSA–ARS complex was formed and gradually unfolded by urea from 0 to 8.0M. The unfolding occurred in different...
An efficient way was proposed for probing the folding/unfolding event of bovine hemoglobin (Hb) through adsorptive-transfer voltammetry. Hb molecules in native and pre-unfolded in different urea conditions for 23h were adsorbed onto the montmorillonite clay modified glassy carbon electrode (Hb/clay/GCE and uHb/clay/GCE, respectively). Cyclic voltammograms of Hb/clay/GCE and uHb/clay/GCE showed that...
The electrochemical behaviors of hemoglobin (Hb) were studied by adsorbing Hb on the gold electrodes modified with self-assembled monolayers (SAMs) of different terminal groups and alkyl chain length. Specifically, through adsorbing the Hb molecules onto the SAMs of 3-mercaptopropanoic acid (MPA), 1-propanethiol (PT) and cysteamine hydrochloride (cys), the influences of the terminal groups of alkanethiols...
A new strategy for fabricating a sensitivity-enhanced glucose biosensor was presented, based on multi-walled carbon nanotubes (CNT), Pt nanoparticles (PtNP) and sol–gel of chitosan (CS)/silica organic–inorganic hybrid composite. PtNP-CS solution was synthesized through the reduction of PtCl 62− by NaBH 4 at room temperature. Benefited from the amino groups of CS, a stable PtNP...
In this paper, the support vector machine was trained to grasp the relationship between the pair-coupled amino acid composition and the content of protein secondary structural elements, including α-helix, 3 10 -helix, π-helix, β-strand, β-bridge, turn, bend and the rest random coil. Self-consistency and cross validation tests were made to assess the performance of our method. Results superior...
In this paper, continuous wavelet transform (CWT) is used to extract the number and the relevant positions of the α-helices and short peptides connecting α-helices and β-strands (connecting peptides) from the amino acid sequences of proteins. The amino acid sequence is first mapped into hydrophobicity sequence, and then transformed into CWT value of sequence domain in appropriate scale via CWT. The...
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