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Human growth hormone 20kDa (20kDa-hGH) is a natural variant of the main hGH isoform (22kDa-hGH). Since some 20- and 22kDa-hGH biological activities are not identical, we decided to map the prolactin (PRLR) and growth hormone receptor (GHR) binding sites for both isoforms. Monoclonal antibody (MAb) R7B4, directed to both receptors, was employed to estimate the relative proximity between 20- and 22kDa-hGH...
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