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Soybean cell suspension cultures have been used to investigate the role of the elevation of the cytosolic Ca 2+ concentration in β-glucan elicitors-induced defence responses, such as H 2 O 2 and phytoalexin production. The intracellular Ca 2+ concentration was monitored in transgenic cells expressing the Ca 2+ -sensing aequorin. Two lines of evidence...
Abstract. Transgenic soybean (Glycine max L.) cells expressing aequorin were used to monitor changes in cytosolic Ca2+ concentrations in response to treatment with fungal elicitors. After an apparent lag phase of about 60s, both chitin fragments and -glucan elicitors caused a rapid increase in cytosolic Ca2+ concentration, which peaked within 22.5min of treatment. The Ca2+ concentration then decreased...
A unique glucan has been isolated from a recombinant strain of a Rhizobium meliloti TY7, a cyclic β-(1 → 2) glucan mutant carrying a locus specifying β-(1 → 3; 1 → 6) glucan synthesis from Bradyrhizobium japonicum USDA110. This compound, which appears to have considerable hydrophobic affinity, was separated from a perchloric acid cell extract by adsorption to a C-18 silica column. Unlike those cyclic...
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