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Ribosomal protein S9 is a component of the 40S ribosomal submit, encoded by the RPS9 gene in eucaryotic cell. It belongs to the S4P family of ribosomal proteins. To explore the structure characteristic of RPS9 gene of giant panda (Ailuropoda melanoleuca), primers were designed according to the known nucleotide sequence of RPS9 genes to clone the cDNA and genomic sequences of RPS9 gene of A. melanoleuca...
Ribosomal protein S3A (RPS3A) is a component of the 40s small ribosomal subunit, which plays an important role in ribosome. To explore the structure characteristic of RPS3A gene of giant panda (Ailuropoda Melanoleuca), the cDNA and the genomic sequence of RPS 3A were cloned successfully from the giant panda by RT-PCR and Touch-down technology respectively, followed by sequencing and analysing. The...
Ribosomal protein L10A is a component of the 60S large ribosomal subunit. To explore the structure feature of RPL10A gene of the Ailuropoda melanoleuca,the cDNA and the genomic sequence of RPL10A gene were cloned using RT-PCR and touchdown -PCR technology. The obtained cDNA of RPL10A in giant panda is 689 bp in size, containing an ORF of 654 bp, and the deduced protein was composed of 217 amino acids...
To explore the structure characteristic of rpL13 gene of Ailuropoda melanoleuca, RT-PCR and PCR strategy were employe d to isolate cDNA and genomic sequence of this gene from A. melanoleuca respectively, followed by sequencing and analyz ing preliminarily. The results indicated that the length of cDNA fragment cloned was 660 bp, containing an open reading frame of 636 bp, encoding 211 amino acids...
To explore the structure characteristic of rpL14 gene of A. melanoleuca and the differences among other species reported, we have designed primers based on the known information of rpL14 gene. Then, the cDNA and the genomic sequence of rpL14 gene were cloned successfully from A. melanoleuca by RT-PCR and PCR strategy respectively, and followed by sequencing and analyzing. The result indicated that...
40S ribosomal protein S10 is a protein encoded by the RPS10 gene in human, belonging to the S10E family of ribosomal proteins. To explore the structure characteristic of this gene of Ailuropoda melanoleuca, primers were designed based on the known nucleotide sequence of RPS10 gene. The RT-PCR and PCR strategy were employed to isolate cDNA and genome of RPS10 from A. melanoleuca respectively, followed...
The rpl18 is a component of the 60s large ribosomal subunit playing an important role in ribosome. Based on the known nucleotide sequence of rpl18 genes, primers were designed to clone cDNA and genomic sequence of rpl18 gene from giant panda (Ailuropoda melanomleuca) successfully, and the structure characteristic of this gene was analyzed. The cDNA fragment of the rpl18 cloned from A. melanoleuca...
Ribosomal protein S18 is a component of the 40S subunit, encoded by the RPS18 gene in eucaryotic cell, belonging to the S13P family of ribosomal proteins. To explore the structure characteristic of RPS18 gene of the Ailuropoda melanoleuca, primers we re designed based on the known nucleotide sequence of RPS18 gene to clone the cDNA and genomic sequences of RPS18 gene of A. melanoleuca by RT-PCR and...
RPS5 is a component of the 40S small ribosomal subunit encoded by rpS5 gene, which is specific to eukaryotes. The cDNA and the genomic sequence of rpS5 were cloned successfully from giant panda using RT-PCR technology and Touchdown-PCR, respectively. Both sequences were analyzed preliminarily and the cDNA of the rpS5 gene was overexpressed in E. coli BL21. The cDNA of rpS5 cloned from giant panda...
Powerful methods for integrated analysis of multiple biological data sets must be developed to maximize researchers' ability to interpret them and acquire meaningful knowledge. Projection onto the most interesting statistical evidence (PROMISE) is a powerful statistical procedure that has been recently developed to incorporate a biological paradigm for the relationships among endpoint variables into...
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