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Thirty-two Holstein heifers with body weights (BW) between 213 and 231kg were randomly assigned to one of four treatments for the 50-d trial. Treatments consisted of four percentages of rumen-undegradable protein (RUP) (31, 43, 50, and 55% of total N) at 100% of National Research Council recommendations for total digestible nutrients and crude protein. Total mixed diets composed of corn silage, ground...
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