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Corynebacterium glutamicum is an important microorganism for production of amino acids in industrial fermentation. Suitable vectors are needed for metabolic engineering in C. glutamicum. Most available vectors used in C. glutamicum carry antibiotic resistant genes as a genetic labeling for rapid identification of recombinant strains, and antibiotics have to be added to maintain the vector when growing...
Corynebacterium glutamicum is an industrial microorganism for production of amino acids. However, the metabolic engineering in C. glutamicum has been retarded due to lack of suitable vectors. In this study, we have constructed a shuttle vector pDXW-10 which harbors a large multiple cloning site suitable for cloning multiple genes, and a tac-M promoter suitable for constitutive gene expression in C...
Brevibacterium flavum has been developed to produce amino acids l-valine, l-lysine and l-threonine. However, there are not enough vectors available for the research on metabolic engineering in Brevibacterium flavum. Here we have constructed a shuttle expression vector pDXW-8 between Escherichia coli and B. flavum. The vector harbors an origin oriE for replicating in E. coli, a second origin oriC for...
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