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In order to reconstruct the system for identification of short antigenic peptides, the chicken BF2 gene of Chinese Sanhuang (SH) chicken line was linked to the β 2 m gene via (G4S)3, a linker encoding a 15-amino acid glycine-rich peptide, by splicing overlap extension PCR (SOE-PCR). The MBP–BF2–(G4S)3–β 2 m fusion protein was expressed and purified in a pMAL-p2X/E. coli TB1 system...
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