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Escherichia coli accumulates K + by means of multiple uptake systems of which Kup is the major transport system at acidic pH. In cells grown under fermentative conditions at pH 5.5, K + influx by a wild-type strain upon hyper-osmotic stress at pH 5.5 was accompanied by a marked decrease in H + efflux, with a 1:1 ratio of K + to H + fluxes. This was observed...
The K + uptake was observed in washed cells of Escherichia coli, wild-type, upon hyper-osmotic stress at pH 5.5 when glucose was supplemented. This uptake had apparent a K m of 0.58 mM and V max of 0.10 μmol K + /min/mg protein. Such a K + uptake was investigated using a mutant defective in Kdp and TrkA but with a functional Kup and a mutant defective...
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