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Recently we identified a so far unique protein (CHB1) which interacts specifically with crystalline α-chitin. Having optimized the binding conditions for CHB1 coupled with fluorescein isothiocyanate (FITC), we succeeded in developing a highly sensitive assay to detect α-chitin. CHB1-FITC interacted neither with β- or colloidal chitin nor with chitooligomers or cellulose. With the help of fluorescence...
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