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The uptake of colicin U into sensitive cells is dependent on the Tol system. Like colicin A and the E-type colicins, colicin U contains the consensus pentapeptide DG(T/S)G(S/W) in the N-terminal 36 amino acids, which have been proposed to be involved in the interaction of colicin U with the Tol proteins. The role of the DGTGW motif in colicin U was studied by converting it into the TonB box motif...
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