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Ligationen: Die Immunoglobin(Ig)‐Domäne von Tim‐3 wurde chemoenzymatisch synthetisiert. Die Domäne wurde in drei Segmente geteilt, und die N‐ und S‐geschützten Peptidthioester wurden an der Festphase synthetisiert. Die Eintopf‐Ligationen wurden durch Ag+‐freie und Ag+‐unterstützte Aktivierung der Thioester erreicht. Die Faltung und der enzymatische Transfer des synthetischen Glycans führten zur Nonasaccharid‐tragenden...
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