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Trypsins have been purified and characterized in a multitude of mammalian and nonmammalian species, but not in the domestic cat (Felis catus). In several species two or more isoforms of trypsin have been isolated. Feline trypsin was purified from feline pancreas by sulfuric acid extraction, ammonium sulfate fractionation, gel filtration and affinity chromatography on a benzamidine-activated sepharose...
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