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A preparative high-speed counter-current chromatography (HSCCC) method for isolation and purification of flavonoids from the Chinese medicinal plant Epimedium koreamum Nakai was successfully established by using chloroform–methanol–water (4:3.5:2, v/v) as the two-phase solvent system. The method yielded 11.4mg of epimedokoreanoside I, 46.5mg of icariin and 17.7mg of icariside II from 200mg of the...
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