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In this paper, thermally reversible hydrogel used as a replaceable packed material for capillary electrophoresis (CE) was examined. A simple and rapid method for detecting estradiol (E2) was developed, which used a strong affinity antibody (K d =1.5x10 9 ) as a selector for immunologically based separations in serum by CE, using an uncoated capillary. Monoclonal antibody (McAb) was...
A new mimetic enzyme immunoassay (MEIA) method for α-1-fetoprotein (AFP) was developed using a mimetic enzyme Mn(III)-tetra(sulfophenyl)porphine (Mn-TPPS 4 ) as a labelling reagent to catalyze the fluorescence reaction of 4-hydroxyphenylacetic acid (HPA) and hydrogen peroxide. In the sandwich assay standard AFP solution or AFP containing umbilical blood serum first reacts with antibody (anti-AFP)...
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