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To study the effects of interleukin-6 (IL-6) and its receptor (IL-6R) at each stage in the early development of in vitro-fertilized (IVF) sheep embryos, the mRNA and protein expression levels of IL-6 and IL-6R were evaluated along with their localization, through real-time PCR, protein blotting, and immunohistochemistry. Specific patterns of IL-6 and IL-6R expression were recorded at both the mRNA...
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