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The rice cultivar Zhejing 22 has been transformed to carry the Bacillus thuringiensis Cry1A(b) gene in 2007. Although the cultivation of this line (referred to as Bt-ZJ22) is yet to be authorized, it is likely to be approved in China in the near future. The transgene-host 3’ integration junction was isolated using thermal asymmetric interlaced PCR, and its sequence was used to design PCR primers and...
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