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This chapter contains sections titled:
Introduction
Single Cell Gene Expression Profiling
Fluorescence‐Activated Cell Sorting
Live Cell Fluorescence Microscopy
Other Techniques for Single Cell Analysis
Conclusions and Outlook
References
Cardiac fibroblasts regulate tissue repair and remodeling in the heart. To quantify transcript levels in these cells we performed a comprehensive gene expression study using serial analysis of gene expression (SAGE). Among 110,169 sequenced tags we could identify 30,507 unique transcripts. A comparison of SAGE data from cardiac fibroblasts with data derived from total mouse heart revealed a number...
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