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2-Amino-3-phenylpyrazine is found to be a sensitive fluorescence labeling reagent for saccharides with a reducing end. The labeled monosaccharides show strong fluorescence under various pH conditions, and could be analyzed by both HPLC and HPCE techniques. Laser induced fluorescence detection is also applicable. Following derivatization with 2-amino-3-phenylpyrazine, six monosaccharides are separated...
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