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Human tumor necrosis factor a (hTNFa) was found to increase rainbow trout macrophage respiratory burst activity and neutrophil migration. The increase in respiratory burst activity was inhibited by preincubation of the hTNFa with a soluble 55 kDa hTNFa receptor (TNF-Rl). Preincubation of the phagocytes with monoclonal antibodies (MAbs) to the TNF-R1 was found to ablate their responsiveness to hTNFa,...
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