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Immunodeficient NOD-scid mice bearing a targeted mutation in the IL2 receptor common gamma chain (Il2rγ null ) readily engraft with human stem cells. Here we analyzed human peripheral blood mononuclear cells (PBMC) for their ability to engraft NOD-scid Il2rγ null mice and established engraftment kinetics, optimal cell dose, and the influence of injection route. Even at low PBMC input,...
Background. Treatment with a single donor-specific transfusion (DST) plus a brief course of anti-CD154 monoclonal antibody (mAb) prolongs skin allograft survival in mice. It is known that prolongation of allograft survival by this method depends in part on deletion of alloreactive CD8 + T cells at the time of tolerance induction. Recent data suggest that infection with lymphocytic choriomeningitis...
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