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We have developed a novel regulated expression vector for Bacillus subtilis based on the Staphylococcus aureus plasmid pUB110. This vector, named pPR54, carries the P R promoter and the cI857 gene (encoding a temperature-sensitive transcriptional repressor) from the Escherichia coli phage λ. Using the gfp gene from the jellyfish Aequorea victoria as a reporter, we show that pPR54 is a useful...
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