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A method for micropropagation of the protected species Gentiana pneumonanthe L. by somatic embryogenesis (SE) was elaborated. For SE induction, secondary explants of leaves and apical meristems from in vitro cultures were successfully used. Tests of callus induction were carried out on ten media containing BA (0.04-8.0 µM) and Picloram or 2,4-D (4.0-8.0 µM). The greatest amounts of embryogenic callus...
Shoot and bulb induction of hyacinths under the influence of varying light treatments (blue, green, yellow, red, far-red and UV irradiation) was studied in long-term in vitro cultures. Leaf explants were cultivated on solid MS medium containing sucrose or glucose (90, 180 or 360 mM). The light as well as the types and concentrations of carbohydrates affected regeneration. Blue light stimulated growth...
Different explants (root and hypocotyl slices or leaf parts) isolated from aseptic seedlings of Cyclamen persicum Mill. F1 ‘Medium’ were exposed to various concentrations of auxins and cytokinins. On the media with a higher concentration of cytokinin (2iP at 25 µM) than auxin (NAA at 2.5 µM) all the explants produced low amounts of callus or else directly generated adventitious roots as well as shoots...
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