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A method is described for the indirect determination of trimethylamine oxide (TMAO) in small volumes of seawater (300 μl) and in seafood tissue extracts (100 μl). After simple TMAO reduction to trimethylamine (TMA), the samples were directly injected into the flow injection system enabling the TMAO to be measured as TMA. Calibration of the method with acidic TMAO standard solutions in the range...
Increased trimethylamine (TMA) levels in tissues are considered to be characteristic of marine fish spoilage. A direct method has been developed for the determination of TMA in aqueous solutions and in fish muscle extracts, which involves the extraction of a small quantity of tissue (1 g) in perchloric acid and the subsequent analysis of the extract by an inexpensive flow injection/gas-diffusion...
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