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Here, a gene‐edited single clone of a cancer cell line, isolated in a droplet, is being pulled out of a microfluidic trap. By combining digital microfluidics and a channel architecture, a single cell is trapped and encapsulated in article number 2002400 by Steve C. C. Shih and co‐workers. With the click of a button, droplet movements can be performed, sorting out the clone for recovery off the chip...
Generating a stable knockout cell line is a complex process that can take several months to complete. In this work, a microfluidic method that is capable of isolating single cells in droplets, selecting successful edited clones, and expansion of these isoclones is introduced. Using a hybrid microfluidics method, droplets in channels can be individually addressed using a co‐planar electrode system...
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