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The pituitary growth hormone (GH) gene of silver sea bream (Sparus sarba) was cloned and characterized and found to be 615 base pairs encoding a protein of 204 amino acids. Using a bacterial expression system, recombinant protein was prepared and rabbit polyclonal antibody was raised. Transcript and protein amounts of GH were measured in fish that were adapted to a range of salinities, acclimated...
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