Search results for: Martin Flajšhans

Items from 1 to 20 out of 37 results

article

How do suboptimal temperatures affect polyploid sterlet Acipenser ruthenus during early development?

Martin Hubálek, Vojtěch Kašpar, Tomáš Tichopád, Marek Rodina, more

Journal of Fish Biology > 101 > 1 > 77 - 91

Sturgeons are ancient fish exhibiting unique genome plasticity and a high tendency to produce spontaneously autopolyploid genome states. The temperature profiles of the rivers in which sturgeon live and reproduce have been severely altered by human intervention, and the effect of global warming is expected to cause further temperature shifts, which may be detrimental for early developmental stages...

article

Simple Field Storage of Fish Samples for Measurement of DNA Content by Flow Cytometry

Martin Hubálek, Martin Flajšhans

Cytometry Part A > 99 > 7 > 743 - 752

Flow cytometry is an effective and widely used tool for determination of ploidy in fish, but it is not always possible to access the fresh samples for analysis. We investigated the potential for extended storage of fish tissue with sterlet and tench as representative species of Chondrostei and Teleostei, using blood and fin of subadult/adult specimens and tail of larvae. Thirteen procedures for extending...

article

The heterosis estimates for growth and survival traits in sterlet and Siberian sturgeon purebreds and hybrids

Sahana Shivaramu, Doi Thi Vuong, Miloš Havelka, Ievgen Lebeda, more

Journal of Applied Ichthyology > 36 > 3 > 267 - 274

The aim of the present study was to estimate and compare the growth and survival traits of the hybrids and purebreds produced by crossing the Siberian sturgeon (Acipenser baerii) (S) and sterlet (Acipenser ruthenus) (St) in order to determine the heterosis effect in the F1 generation. We compared the breeding conditions, mean body weight (BW) and cumulative survival in the artificially produced hybrid...

article

Critical swimming speed of sterlet (Acipenser ruthenus): Does intraspecific hybridization affect swimming performance?

Sahana Shivaramu, Carlos E. Santo, Vojtěch Kašpar, David Bierbach, more

Journal of Applied Ichthyology > 35 > 1 > 217 - 225

We studied the effect of intraspecific hybridization on swimming performance in sterlet, hypothesizing that such hybridization increases the performance by inducing the hybrid vigor. A total of 12 purebred and hybrid crosses were reproduced from Danube (D) and Volga (V) populations of Acipenser ruthenus. Within each cross, one group of fish was exposed to temperature challenges mimicking the temperature...

article

Egg oxidation status, antioxidant enzyme activities, lipid classes, fatty acid composition profile and embryo survival rates during in vitro oocyte ageing in tench Tinca tinca (Linnaeus, 1758)

Azadeh Mohagheghi Samarin, Sabine Sampels, Anna Krzyskow, Viktoriia Burkina, more

Aquaculture Research > 49 > 6 > 2305 - 2316

The effect of in vitro storage of oocytes on embryos survival rate, egg oxidation status, antioxidant enzyme activities, lipid classes and fatty acid composition profile was investigated in common tench Tinca tinca. In order to identify the role of oxidative stress in the progress of oocyte ageing the levels of thiobarbituric acid reactive substances (TBARS) and carbonyls as indicators of lipid and...

article

Molecular cytogenetic differentiation of paralogs of Hox paralogs in duplicated and re-diploidized genome of the North American paddlefish (Polyodon spathula)

Radka Symonová, Miloš Havelka, Chris T. Amemiya, William Mike Howell, more

BMC Genetics > 2017 > 18 > 1 > 1-12

Background Acipenseriformes is a basal lineage of ray-finned fishes and comprise 27 extant species of sturgeons and paddlefishes. They are characterized by several specific genomic features as broad ploidy variation, high chromosome numbers, presence of numerous microchromosomes and propensity to interspecific hybridization. The presumed palaeotetraploidy of the American paddlefish was recently validated...

article

Influence of photoreactivation on induction of gynogenesis in sterlet, Acipenser ruthenus

Ievgen Lebeda, Martin Flajshans

Aquaculture Research > 47 > 4 > 1364 - 1368

article

The second highest chromosome count among vertebrates is observed in cultured sturgeon and is associated with genome plasticity

Miloš Havelka, Dmytro Bytyutskyy, Radka Symonová, Petr Ráb, more

Genetics Selection Evolution > 2016 > 48 > 1 > 1-9

Background One of the five basal actinopterygian lineages, the Chondrostei, including sturgeon, shovelnose, and paddlefish (Order Acipenseriformes) show extraordinary ploidy diversity associated with three rounds of lineage-specific whole-genome duplication, resulting in three levels of ploidy in sturgeon. Recently, incidence of spontaneous polyploidization has been reported among cultured sturgeon...

article

Post-ovulatory and post-stripping oocyte ageing in northern pike, Esox lucius (Linnaeus, 1758), and its effect on egg viability rates and the occurrence of larval malformations and ploidy anomalies

Azin Mohagheghi Samarin, Miroslav Blecha, Mariia Uzhytchak, Dmytro Bytyutskyy, more

Aquaculture > 2016 > 450 > C > 431-438

To identify the effects of oocyte ageing on egg quality in northern pike, Esox lucius, two experiments were performed as follows: in experiment I, partial volumes of the eggs from 7 pike females were stripped and fertilised with 2-day intervals for 8days post-ovulation (DPO). In experiment II, the stripped eggs of 3 females were stored in ovarian fluid at 10°C and fertilised separately with 12-hour...

article

Experience with fish sperm cryobanking in the Czech Republic

Martin Flajshans, Marek Rodina, Otomar Linhart, Borys Dzyuba, more

Cryobiology > 2015 > 71 > 3 > 556

article

The seasonal changes of innate immunity of tench, Tinca tinca (L.) with different ploidy level

Soňa Tolarová, Martina Dávidová, Andrea Šimková, Martin Flajšhans, more

Aquaculture > 2014 > 432 > Complete > 46-52

The study analysed the effect of fish ploidy level and seasonality on haematological parameters and innate immune response of tench (Tinca tinca, Cyprinidae), fish species extensively bred in European aquaculture. Fish samples were collected from a breeding pond in Vodňany (Czech Republic) during four sampling periods (September, December, March and June). All measured haematological parameters (erythrocyte...

article

3‐D structure, volume, and DNA content of erythrocyte nuclei of polyploid fish

Dmytro Bytyutskyy, Vitaliy Kholodnyy, Martin Flajšhans

Cell Biology International > 38 > 6 > 708 - 715

We have explored the potential relationship between ploidy level, DNA content (pg DNA nucleus⁻¹), and dimensional characteristics, such as volume (μm³), surface area (μm²), and 3‐D structure of erythrocyte nuclei in a series of fish ploidy level models using Feulgen image analysis densitometry, flow cytometry, and confocal laser scanning microscopy. The species were diploid tench (Tinca tinca) (2n),...

article

Sperm morphology, motility, and velocity in naturally occurring polyploid European weatherfish (Misgurnus fossilis L.)

Sayyed Mohammad Hadi Alavi, Bořek Drozd, Azadeh Hatef, Martin Flajšhans

Theriogenology > 2013 > 80 > 2 > 153-160

Spontaneous polyploidy has been frequently documented in various fish species. This process may lead to disruption in testicular development and function. In the present study, sperm morphology and motility, elements critical to male fertility, were characterized in the naturally occurring triploid (3n) and tetraploid (4n) European weatherfish, Misgurnus fossilis L. (Teleostei, Cobitidae) inhabiting...

article

The seasonal changes in innate immunity of the common carp (Cyprinus carpio)

Soňa Buchtíková, Andrea Šimková, Karolína Rohlenová, Martin Flajšhans, more

Aquaculture > 2011 > 318 > 1-2 > 169-175

The innate immune response in fishes includes both cellular (phagocytes) and humoral (complement system mainly) components. In fish, as in mammals, reactive oxygen metabolites (ROM) are involved in the respiratory burst of phagocytes and three pathways of complement activation can be discerned. The aim of this study was to analyze the innate immune response of fish using parameters such as respiratory...

article

Teleost maturation-inducing hormone, 17,20β-dihydroxypregn-4-en-3-one, peaks after spawning in Tinca tinca

Efthimia Antonopoulou, Athanassios C. Tsikliras, Martin Kocour, Vladimir Žlábek, more

General and Comparative Endocrinology > 2011 > 172 > 2 > 234-242

During an eight month study of the reproductive cycle in two age groups, and in both sexes, of tench (Tinca tinca L.), it was found that plasma concentrations of the presumptive ‘maturation inducing hormone (MIH)’ 17,20β-dihydroxypregn-4-en-3-one (17,20β-P) did not reach a peak during the spawning season, but as much as two months after spawning had ceased. The cessation of the spawning season was...

article

The influence of ploidy level on ultrastructure and motility of tench Tinca tinca (L.) spermatozoa

Martin Pšenička, Martin Flajšhans, Martin Hulák, Vojtěch Kašpar, more

Reviews in Fish Biology and Fisheries > 2010 > 20 > 3 > 331-338

The ultrastructure of diploid and triploid tench Tinca tinca (L.) spermatozoa were examined using electron microscopy focusing on parameters that influence movement. Triploid tench were produced artificially using a cold shock. Spermatozoa of triploid males in comparison with diploids featured significantly larger head (P < 0.01), higher amount of mitochondria (P < 0.05), and, surprisingly larger...

article

Performance of different tench, Tinca tinca (L.), groups under semi-intensive pond conditions: it is worth establishing a coordinated breeding program

Martin Kocour, David Gela, Marek Rodina, Martin Flajšhans

Reviews in Fish Biology and Fisheries > 2010 > 20 > 3 > 345-355

Two trials of the performance (growth, survival, dress out) of a number of different tench breeds/groups were conducted to elucidate the implications for the establishment of a coordinated breeding program. The first trial with a top-crossing scheme had to be concluded after 1st wintering. Age 1 + hybrids of Romanian-R breed with Tabor-T, Vodnany-V and Hungarian-H breeds displayed significantly better...

article

Genetic variability and differentiation of wild and cultured tench populations inferred from microsatellite loci

Klaus Kohlmann, Petra Kersten, Remigiusz Panicz, Devrim Memiş, more

Reviews in Fish Biology and Fisheries > 2010 > 20 > 3 > 279-288

Nine species-specific microsatellites were used to characterize 792 tench, Tinca tinca (L.), from 21 wild and cultured populations. Seven loci were polymorphic expressing four to 22 alleles. A Spanish cultured strain was homozygous at all loci for all individuals studied. Low variability was also observed in a wild population from Sapanca Lake, Turkey and a Chinese cultured strain. In contrast, the...

article

A review on the potential of triploid tench for aquaculture

Martin Flajšhans, David Gela, Martin Kocour, Hana Buchtová, more

Reviews in Fish Biology and Fisheries > 2010 > 20 > 3 > 317-329

Performance and physiological traits and health of spontaneous and induced triploid tench are reviewed. Triploidy is best induced with cold shock; with triploids exhibiting 13.5–51.5% better weight gain, 2.69–3.94% higher slaughtering value, 20–60% lower gonadosomatic index, 0.9–4.5% higher dry matter in flesh and up to 107% more flesh fat than diploids, if farmed untill post sexual maturity. Triploids...

article

Comparison of performance of genome manipulated and standard tench, Tinca tinca (L.), groups under pond management conditions

David Gela, Martin Kocour, Martin Flajšhans, Otomar Linhart, more

Reviews in Fish Biology and Fisheries > 2010 > 20 > 3 > 301-306

A triennial performance test of five groups of tench, Tinca tinca (L.): Vodnany induced triploid V3n, Vodnany meiotic gynogenic Vgyn, diploid Vodnany V2n, Hungarian H2n and German G2n) in pond monoculture was carried out with diploid golden tench as control (C) to compute corrected weight of the groups tested. Survival rate ranged from 11.6% (V2n) to 30.6% (Vgyn) in yearlings, from 71.2% (V2n) to...

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Search results for: Martin Flajšhans

How do suboptimal temperatures affect polyploid sterlet Acipenser ruthenus during early development?

Simple Field Storage of Fish Samples for Measurement of DNA Content by Flow Cytometry

The heterosis estimates for growth and survival traits in sterlet and Siberian sturgeon purebreds and hybrids

Critical swimming speed of sterlet (Acipenser ruthenus): Does intraspecific hybridization affect swimming performance?

Egg oxidation status, antioxidant enzyme activities, lipid classes, fatty acid composition profile and embryo survival rates during in vitro oocyte ageing in tench Tinca tinca (Linnaeus, 1758)

Molecular cytogenetic differentiation of paralogs of Hox paralogs in duplicated and re-diploidized genome of the North American paddlefish (Polyodon spathula)

Influence of photoreactivation on induction of gynogenesis in sterlet, Acipenser ruthenus

The second highest chromosome count among vertebrates is observed in cultured sturgeon and is associated with genome plasticity

Post-ovulatory and post-stripping oocyte ageing in northern pike, Esox lucius (Linnaeus, 1758), and its effect on egg viability rates and the occurrence of larval malformations and ploidy anomalies

Experience with fish sperm cryobanking in the Czech Republic

The seasonal changes of innate immunity of tench, Tinca tinca (L.) with different ploidy level

3‐D structure, volume, and DNA content of erythrocyte nuclei of polyploid fish

Sperm morphology, motility, and velocity in naturally occurring polyploid European weatherfish (Misgurnus fossilis L.)

The seasonal changes in innate immunity of the common carp (Cyprinus carpio)

Teleost maturation-inducing hormone, 17,20β-dihydroxypregn-4-en-3-one, peaks after spawning in Tinca tinca

The influence of ploidy level on ultrastructure and motility of tench Tinca tinca (L.) spermatozoa

Performance of different tench, Tinca tinca (L.), groups under semi-intensive pond conditions: it is worth establishing a coordinated breeding program

Genetic variability and differentiation of wild and cultured tench populations inferred from microsatellite loci

A review on the potential of triploid tench for aquaculture

Comparison of performance of genome manipulated and standard tench, Tinca tinca (L.), groups under pond management conditions

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