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A new electrochemiluminescence testosterone immunoassay (Elecsys® Testo II) was evaluated.Within-run precision, total precision, and method comparisons to the former immunoassay and LC-MS/MS were investigated. Reference intervals were established for adults and children.Imprecision ranged from 0.9% to 8.5%. Method comparisons revealed slopes from 0.68 to 1.32 and coefficients of correlation from 0...
The determination of steroids is important for the diagnosis and monitoring of endocrine diseases and infertility workup. We developed a rapid and reliable mass spectrometric method for the simultaneous quantification of steroid patterns in human serum.An on-line solid phase extraction (SPE)-liquid chromatography–triple quadrupole linear ion trap (LC-QTrap) method utilizing atmospheric pressure chemical...
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