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A23187, a calcium ionophore, is used to induce Ca 2+ -dependent cell death by increasing intracellular Ca 2+ concentration ([Ca 2+ ] i ) under in vitro condition. Since this ionophore also increases membrane permeability of metal divalent cations such as Zn 2+ and Fe 2+ rather than Ca 2+ , trace metal cations in cell suspension may affect Ca...
The effects of polysorbate 80, a non-ionic surfactant widely used in pharmaceutical products, on rat thymocytes were examined to reveal its toxic property at the cellular level. Polysorbate 80 at concentrations of 1-100μg/ml did not significantly affect the cell viability. This surfactant at 30μg/ml or more augmented the intensity of fluo-3 fluorescence, indicating the increase in intracellular Ca...
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