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A highly sensitive method for detection of antigens on the surface of a single blood cell using thermal lens microscopy is described. Colloidal gold, coated with antibody, was used to stain membrane antigens of leukocytes. Human leukocyte antigens on the lymphocytes and mononuclear leukocytes were observed by new thermal lens microscopy, which involves spectrometry using a laser-induced thermal-lens...
Lewis blood group antigen levels in human sera were assayed with a highly sensitive photothermal immunoassay which is based on laser-induced photothermal detection. Comparison of 32 colon cancer patients' sera and 34 healthy persons' sera showed that cancer patients' sera contained more Lewis antigens than healthy persons' sera. Le a antigen level in Le(a-) type persons and Le b level...
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