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Summary form only given. Biological applications of pulsed electric fields (PEF) have been discussed in the last decade. Schoenbach et al have investigated the biological activity of various kinds of cells subjected to nanosecond pulsed electric fields (nsPEF). They reported nsPEF induces the increase in intracellular Ca2+, DNA fragmentation, activation and inactivation of proteins related to apoptosis...
Summary form only given. There are a number of studies that show the biological effects of intense pulsed electric fields (PEFs) dependent on the waveform of the pulses such as duration, rise time as well as the number of pulses. PEFs with a pulse duration exceeding 1 us cause the increase in the permeability of cell membrane in other words electroporation. As the duration and rise time of PEF are...
Intracellular DNA damages caused by intense burst sinusoidal electric fields (IBSEFs) were investigated by means of an alkaline comet assay method. Non-thermal, 200 mus-long IBSEF with various frequency values (300 kHz-100 MHz) and strengths (up to 200 kV/m) was applied to Chinese hamster ovary (CHO) cells in a suspending medium between 1 mm gap parallel electrodes. The comet assay suggests that 100...
This paper describes the effect of intense burst RF electric fields (IBRFFs) on DNA in living mammalian cells. Chinese hamster ovary (CHO) cells were placed between two parallel plane electrodes and exposed to the IBRFF with a MHz range frequency and an electric field strength of a couple of kV/cm. The IBRFF duration was fixed to 100 ps, which was sufficiently short so as to not raise the temperature...
Burst RF fields (BRFFs) were applied to Chinese Hamster Ovary (CHO) cells, and the cell response to the BRFF of two different frequencies, 20 kHz and 50 MHz, was investigated by means of the fluorescent microscopy. The field strength and duration of the BRFF were fixed at 1 kV/cm, and 100 mus, respectively. Cells were placed in a 100 mum-gap electrode plated on the slide glass for a microscopy. Acridine...
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