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By the direct observation of single duplex DNA molecules by fluorescence microscopy, we found that RNA molecules have the potential to change discretely the higher-order structure of individual DNA molecules between the compact and elongated states. We performed an experiment with a linear giant DNA (T4 DNA, 166 kbp) and a circular DNA (cosmid vector, 42 kbp), and examined the effect of single-strand...
We have performed direct observations on the conformational change of a single double-stranded T4 DNA molecule induced by diaminoalkanes, NH +3 (CH 2 ) n NH +3 (n = 1 6), by use of fluorescence microscopy. It was found that diamines with three and five methylene groups show a significant effect on the compaction of individual single DNA molecules. On the...
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