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A selective screening method for detection of gene mutations in highly mutated genes associated with cystic fibrosis is described. The method relies on a disposable DNA electrochemical biosensor based on the use of a selective pentaammine [3‐(2‐phenanthren‐9‐yl‐vinyl)‐pyridine] ruthenium complex as electrochemical indicator. This complex incorporates dual functionalities: the Ru center provides a...
Impedance spectroscopy (EIS) has been proposed as the transduction principle for detecting the hybridization of DNA complementary strands. The resistance offered to the electrochemical reaction acts as the working signal, allowing an unlabelled gene assay. In our experiments, atypically large DNA oligonucleotides (110 base pairs) were used to determine the influence of ionic strength on the response...
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