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Protein L (PpL) is a B-cell superantigen from Peptostreptococcus magnus known to bind to mammalian Vκ light chains. PpL from P.magnus strain 312 comprises five homologous immunoglobulin (Ig) binding domains. We first analysed the binding of the individual domains (B1–B5) of PpL 312 to human Vκ light chains (huVκ) subtypes 1 (huVκI) and 3 (huVκIII). Using a combination of rational design and...
A range of well-defined IgG glycoforms was prepared by employing a combination of synthetic carbohydrate chemistry and genetic engineering. The key aspect of this methodology is the coupling of thioaldoses with cysteine-containing proteins to give disulfide-linked neoglycoproteins. This technology was applied to the synthesis of a series of synthetic N-glycan thioaldoses which were coupled to an aglycosylated...
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