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A sensitive, surface plasmon resonance (SPR)-based assay monitoring potential human–anti-human antibody (HAHA) reactions against the monoclonal antibody (mAb) IGN311 is presented. The latter is a fully humanized Lewis-Y carbohydrate specific mAb that is currently tested in a passive immune therapy approach in a clinical phase I trial.For the SPR experiments a BIACORE 3000 analyzer was used. The ligand...
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