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In this study, we attempted to develop a surface plasmon resonance (SPR)-based immunoassay sensor to detect α-fetoprotein (AFP) in human plasma at the nanogram level, as is required for clinical diagnosis of hepatocellular tumors. A self-assembled monolayer (SAM) surface of tri(ethylene glycol) (TEG) and carboxyl group-terminated hexa(ethylene glycol) (HEG) was employed to suppress the nonspecific...
In this study, we developed a surface plasmon resonance (SPR)-based sensing method with high sensitivity for the detection of brain natriuretic peptide (BNP), the level of which is a useful indicator of heart failure. In our system, BNP was detected by a sandwich-type immunoassay using two kinds of monoclonal antibodies: primary and secondary. The SPR signal was amplified by using streptavidin-conjugated...
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