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2ʹ‐Fucosyllactose (2ʹ‐FL) which is well‐known human milk oligosaccharide was biotechnologically synthesized using engineered Corynebacterium glutamicum, a GRAS microbial workhorse. By construction of the complete de novo pathway for GDP‐L‐fucose supply and heterologous expression of Escherichia coli lactose permease and Helicobacter pylori α‐1,2‐fucosyltransferase, bioengineered C. glutamicum BCGW_TL...
As a renewable energy from biomass, isobutanol is considered as a promising alternative to fossil fuels. To biotechnologically produce isobutanol, strain development using industrial microbial hosts, such as Escherichia coli, has been conducted by introducing a heterologous isobutanol synthetic pathway. However, the toxicity of produced isobutanol inhibits cell growth, thereby restricting improvements...